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OPN1SW Recombinant antibody

OPN1SW Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240714A5

Cat no : 83754-6-RR

Synonyms

Short-wave-sensitive opsin 1, CBT, BOP, Blue-sensitive opsin, Blue sensitive opsin

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse eye tissue using OPN1SW Recombinant antibody (83754-6-RR)

    IHC staining of mouse eye using 83754-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83754-6-RR (OPN1SW antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN1SW Recombinant antibody (83754-6-RR)

    IF Staining of mouse eye using 83754-6-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (83754-6-RR, Clone: 240714A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83754-6-RR

    Affinity and Kinetic Characterization of 83754-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83754-6-RR against Human OPN1SW were performed. The affinity constant is 3.58 nM.

Tested Applications

Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83754-6-RR is OPN1SW Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OPN1SW fusion protein Ag20445
Full Name opsin 1 (cone pigments), short-wave-sensitive
Calculated Molecular Weight 348 aa, 39 kDa
GenBank Accession NumberBC156719
Gene Symbol OPN1SW
Gene ID (NCBI) 611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPN1SW (Short-wave-sensitive opsin 1) belongs to the G-protein coupled receptor 1 family, opsin subfamily. It's one of three types of cone photoreceptors responsible for normal color vision. Inherited tritan color vision deficiencies exhibit an autosomal dominant inheritance pattern and are caused by mutations in OPN1SW (PMID: 32400513).

Protocols

Product Specific Protocols
IHC protocol for OPN1SW antibody 83754-6-RRDownload protocol
IF protocol for OPN1SW antibody 83754-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols