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RAB37 Recombinant antibody

RAB37 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240838D6

Cat no : 83740-4-RR

Synonyms

Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB37 Recombinant antibody (83740-4-RR)

    WB analysis using 83740-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83740-4-RR (RAB37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83740-4-RR

    Affinity and Kinetic Characterization of 83740-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83740-4-RR against Human RAB37 were performed. The affinity constant is 1.25 nM.

Tested Applications

Positive WB detected inJurkat cells, A549 cells, mouse brain tissues, rat brain tissues

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83740-4-RR is RAB37 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB37 fusion protein Ag3717
Full Name RAB37, member RAS oncogene family
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC016615
Gene Symbol RAB37
Gene ID (NCBI) 326624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

Protocols

Product Specific Protocols
WB protocol for RAB37 antibody 83740-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols