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ACOX1 Recombinant antibody

ACOX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240754D3

Cat no : 83731-1-RR

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX1 Recombinant antibody (83731-1-RR)

    WB analysis using 83731-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83731-1-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACOX1 Recombinant antibody (83731-1-RR)

    IF Staining of HepG2 using 83731-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AOX antibody (83731-1-RR, Clone: 240754D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83731-1-RR

    Affinity and Kinetic Characterization of 83731-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83731-1-RR against Human ACOX1 were performed. The affinity constant is 0.777 nM.

Tested Applications

Positive WB detected inHuH-7 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83731-1-RR is ACOX1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACOX1 fusion protein Ag1405
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated Molecular Weight 74 kDa
Observed Molecular Weight70-74 kDa, 22 kDa
GenBank Accession NumberBC010425
Gene Symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Protocols

Product Specific Protocols
WB protocol for ACOX1 antibody 83731-1-RRDownload protocol
IF protocol for ACOX1 antibody 83731-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols