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VCAM-1/CD106 Recombinant antibody

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240597B2

Cat no : 83719-1-RR

Synonyms

CD106, VCAM-1, Vcam1, Vcam 1, 240597B2

Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using VCAM-1 Recombinant antibody (83719-1-RR)

    WB analysis of 3T3-L1 using 83719-1-RR

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83719-1-RR

    Affinity and Kinetic Characterization of 83719-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

Tested Applications

Positive WB detected in3T3-L1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83719-1-RR is VCAM-1/CD106 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name vascular cell adhesion molecule 1
Calculated Molecular Weight81 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberCAA47989.1
Gene Symbol VCAM-1
Gene ID (NCBI) 22329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
WB protocol for VCAM-1/CD106 antibody 83719-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols