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TFF2 Recombinant antibody

TFF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240661G12

Cat no : 83717-4-RR

Synonyms

SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

Validation Data Gallery

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  • Western Blot (WB) analysis of human urine sample using TFF2 Recombinant antibody (83717-4-RR)

    WB analysis of human urine using 83717-4-RR

    Human urine sample was subjected to SDS PAGE followed by western blot with 83717-4-RR (TFF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse stomach tissue using TFF2 Recombinant antibody (83717-4-RR)

    IP experiment of mouse stomach using 83717-4-RR

    IP result of anti-TFF2 (IP:83717-4-RR, 4ug; Detection:83717-4-RR 1:1000) with mouse stomach tissue lysate 1360 ug.

  • Flow cytometry (FC) experiment of PC-3 cells using TFF2 Recombinant antibody (83717-4-RR)

    FC experiment of PC-3 using 83717-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug TFF2 Recombinant antibody (83717-4-RR, Clone:240661G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83717-4-RR

    Affinity and Kinetic Characterization of 83717-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83717-4-RR against Human TFF2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inhuman urine sample
Positive IP detected inmouse stomach tissue
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83717-4-RR is TFF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFF2 fusion protein Ag4537
Full Name trefoil factor 2
Calculated Molecular Weight 129 aa, 14 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC032820
Gene Symbol TFF2
Gene ID (NCBI) 7032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

Protocols

Product Specific Protocols
WB protocol for TFF2 antibody 83717-4-RRDownload protocol
IP protocol for TFF2 antibody 83717-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols