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Beta galactosidase Recombinant antibody

Beta galactosidase Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240788F1

Cat no : 83706-6-RR

Synonyms

GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta galactosidase Recombinant antibody (83706-6-RR)

    WB analysis using 83706-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta galactosidase Recombinant antibody (83706-6-RR)

    IF Staining of HepG2 using 83706-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using Beta galactosidase Recombinant antibody (83706-6-RR)

    FC experiment of A549 using 83706-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83706-6-RR

    Affinity and Kinetic Characterization of 83706-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, SH-SY5Y cells, HEK-293 cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83706-6-RR is Beta galactosidase Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta galactosidase fusion protein Ag7792
Full Name galactosidase, beta 1
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 62-67 kDa
GenBank Accession NumberBC007493
Gene Symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.

Protocols

Product Specific Protocols
WB protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
IF protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
FC protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols