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  • Western Blot (WB) analysis of various lysates using GALNT2 Recombinant antibody (83703-2-RR)

    WB analysis using 83703-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83703-2-RR (GALNT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GALNT2 Recombinant antibody (83703-2-RR)

    IHC staining of human ovary cancer using 83703-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83703-2-RR (GALNT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83703-2-RR

    Affinity and Kinetic Characterization of 83703-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83703-2-RR against Human GALNT2 were performed. The affinity constant is 2.04 nM.

GALNT2 Recombinant antibody

GALNT2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83703-2-RR
Clone No.240723G10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2, EC:2.4.1.41

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MKN-45 cells, A549 cells, U-87 MG cells, HuH-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83703-2-RR targets GALNT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GALNT2 fusion protein Ag11523 Predict reactive species
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
Calculated Molecular Weight 571 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC041120
Gene Symbol GALNT2
Gene ID (NCBI) 2590
RRIDAB_3671305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ10471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

Protocols

Product Specific Protocols
WB protocol for GALNT2 antibody 83703-2-RRDownload protocol
IHC protocol for GALNT2 antibody 83703-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols