Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, MKN-45 cells, A549 cells, U-87 MG cells, HuH-7 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83703-2-RR targets GALNT2 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | GALNT2 fusion protein Ag11523 Predict reactive species |
Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) |
Calculated Molecular Weight | 571 aa, 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC041120 |
Gene Symbol | GALNT2 |
Gene ID (NCBI) | 2590 |
RRID | AB_3671305 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q10471 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for GALNT2 antibody 83703-2-RR | Download protocol |
IHC protocol for GALNT2 antibody 83703-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |