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GBP4 Recombinant antibody

GBP4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240730F11

Cat no : 83699-2-RR

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBP4 Recombinant antibody (83699-2-RR)

    IF Staining of HeLa using 83699-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP4 antibody (83699-2-RR, Clone: 240730F11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83699-2-RR

    Affinity and Kinetic Characterization of 83699-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-2-RR against Human GBP4 were performed. The affinity constant is 1.18 nM.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83699-2-RR is GBP4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GBP4 fusion protein Ag12006
Full Name guanylate binding protein 4
Calculated Molecular Weight 640 aa, 73 kDa
GenBank Accession NumberBC070055
Gene Symbol GBP4
Gene ID (NCBI) 115361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor.

Protocols

Product Specific Protocols
IF protocol for GBP4 antibody 83699-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols