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PTPN4 Recombinant antibody

PTPN4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240739A11

Cat no : 83695-1-RR

Synonyms

PTPN 4, PTPase-MEG1, PTN4, Protein-tyrosine phosphatase MEG1, EC:3.1.3.48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN4 Recombinant antibody (83695-1-RR)

    WB analysis using 83695-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83695-1-RR (PTPN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83695-1-RR

    Affinity and Kinetic Characterization of 83695-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83695-1-RR against Human PTPN4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inrat brain tissue, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83695-1-RR is PTPN4 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTPN4 fusion protein Ag1585
Full Name protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte)
Calculated Molecular Weight 106 kDa
Observed Molecular Weight106 kDa
GenBank Accession NumberBC010674
Gene Symbol PTPN4
Gene ID (NCBI) 5775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN4 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP has been shown to interact with glutamate receptor delta 2 and epsilon subunits, and is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.

Protocols

Product Specific Protocols
WB protocol for PTPN4 antibody 83695-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols