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Alpha-1-microglobulin Recombinant antibody

Alpha 1 microglobulin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240751G7

Cat no : 83678-3-RR

Synonyms

AMBP, Alpha-1-microglobulin, Alpha 1-microglobulin, Alpha 1 microglycoprotein, A1M

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha-1-microglobulin Recombinant antibody (83678-3-RR)

    WB analysis of human plasma using 83678-3-RR

    human plasma were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human urine sample using Alpha-1-microglobulin Recombinant antibody (83678-3-RR)

    WB analysis of human urine using 83678-3-RR

    human urine sample were subjected to SDS PAGE followed by western blot with 83678-3-RR (Alpha-1-microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using Alpha 1 microglobulin Recombinant antibody (83678-3-RR)

    FC experiment of MCF-7 using 83678-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Alpha 1 microglobulin Recombinant antibody (83678-3-RR, Clone:240751G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using Alpha 1 microglobulin Recombinant antibody (83678-3-RR)

    FC experiment of HepG2 using 83678-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha 1 microglobulin Recombinant antibody (83678-3-RR, Clone:240751G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83678-3-RR

    Affinity and Kinetic Characterization of 83678-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83678-3-RR against Human Alpha 1 microglobulin were performed. The affinity constant is 6.59 nM.

Tested Applications

Positive WB detected inhuman plasma, human urine sample
Positive FC (Intra) detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83678-3-RR targets Alpha 1 microglobulin in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name alpha-1-microglobulin/bikunin precursor
Calculated Molecular Weight39kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberNM_001633.4
Gene Symbol Alpha 1 microglobulin
Gene ID (NCBI) 259
RRIDAB_3671281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha 1 microglobulin, also known as HI30, is a 27-30 kDa glycoprotein, present in various body fluids. It belongs to the lipocalin superfamily of hydrophobic ligand-binding proteins forming an internal ligand-binding pocket. The protein acts as a mediator of bacterial adhesion to polymer surfaces and is involved in inhibiting renal lithogenesis.

Protocols

Product Specific Protocols
WB protocol for Alpha 1 microglobulin antibody 83678-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols