Filter: AllWBIHCCytometric bead arrayAffinity

WB analysis using 83674-1-RR (same clone as 83674-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 83674-1-RR (same clone as 83674-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 83674-1-RR (same clone as 83674-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 83674-1-RR (same clone as 83674-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00649-1
Cytometric bead array standard curve of MP00649-1, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-1-PBS. Detection antibody: 83674-2-PBS. Standard: Ag4532. Range: 0.625-80 ng/mL.
Cytometric bead array standard curve of MP00649-2
Cytometric bead array standard curve of MP00649-2, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-4-PBS. Detection antibody: 83674-1-PBS. Standard: Ag4532. Range: 0.625-80 ng/mL.
Cytometric bead array standard curve of MP00649-3
Cytometric bead array standard curve of MP00649-3, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-1-PBS. Detection antibody: 83674-3-PBS. Standard: Ag4532. Range: 1.25-80 ng/mL.
Affinity and Kinetic Characterization of 83674-1-RR
Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

KCNH7 Recombinant antibody, PBS Only (Capture/Detector)

KCNH7 {ptg:Brand} Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 83674-1-PBS

Clone No.240644B5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83674-1-PBS targets KCNH7 as part of a matched antibody pair:

MP00649-1: 83674-1-PBS capture and 83674-2-PBS detection (validated in Cytometric bead array)

MP00649-2: 83674-4-PBS capture and 83674-1-PBS detection (validated in Cytometric bead array)

MP00649-3: 83674-1-PBS capture and 83674-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	KCNH7 fusion protein Ag4532 Predict reactive species
Full Name	potassium voltage-gated channel, subfamily H (eag-related), member 7
Calculated Molecular Weight	1196 aa, 135 kDa
Observed Molecular Weight	135 kDa
GenBank Accession Number	BC035815
Gene Symbol	KCNH7
Gene ID (NCBI)	90134
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NS40
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.