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FTCD Recombinant antibody

FTCD Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240654G9

Cat no : 83664-2-RR

Synonyms

Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formimidoyltetrahydrofolate cyclodeaminase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTCD Recombinant antibody (83664-2-RR)

    WB analysis using 83664-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83664-2-RR (FTCD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using FTCD Recombinant antibody (83664-2-RR)

    FC experiment of HepG2 using 83664-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FTCD Recombinant antibody (83664-2-RR, Clone:240654G9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83664-2-RR

    Affinity and Kinetic Characterization of 83664-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83664-2-RR against Human FTCD were performed. The affinity constant is 6.37 nM.

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83664-2-RR is FTCD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FTCD fusion protein Ag16646
Full Name formiminotransferase cyclodeaminase
Calculated Molecular Weight 541 aa, 59 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC136395
Gene Symbol FTCD
Gene ID (NCBI) 10841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

Protocols

Product Specific Protocols
WB protocol for FTCD antibody 83664-2-RRDownload protocol
FC protocol for FTCD antibody 83664-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols