• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HEATR3 Recombinant antibody

HEATR3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240561F7

Cat no : 83643-4-RR

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3, 240561F7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis using 83643-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83643-4-RR (HEATR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83643-4-RR

    Affinity and Kinetic Characterization of 83643-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83643-4-RR against Human HEATR3 were performed. The affinity constant is 0.657 nM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83643-4-RR is HEATR3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEATR3 fusion protein Ag26328
Full Name HEAT repeat containing 3
Calculated Molecular Weight 680 aa, 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC033077
Gene Symbol HEATR3
Gene ID (NCBI) 55027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HEATR3 antibody 83643-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols