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ATP6V1E1 Recombinant antibody

ATP6V1E1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240584G12

Cat no : 83636-6-RR

Synonyms

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1E1 Recombinant antibody (83636-6-RR)

    WB analysis using 83636-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ATP6V1E1 Recombinant antibody (83636-6-RR)

    IHC staining of human ovary cancer using 83636-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

Tested Applications

Positive WB detected inHepG2 cells, U2OS cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83636-6-RR is ATP6V1E1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP6V1E1 fusion protein Ag7399
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC004443
Gene Symbol ATP6V1E1
Gene ID (NCBI) 529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

Protocols

Product Specific Protocols
WB protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols