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Timp-3 Recombinant antibody

Timp-3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240374D8

Cat no : 83627-1-RR

Synonyms

TIMP3, Protein MIG 5, Metalloproteinase inhibitor 3, K222TA2, K222

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Timp-3 Recombinant antibody (83627-1-RR)

    WB analysis of human placenta using 83627-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83627-1-RR (Timp-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MDA-MB-231 cells using Timp-3 Recombinant antibody (83627-1-RR)

    FC experiment of MDA-MB-231 using 83627-1-RR

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using Timp-3 Recombinant antibody (83627-1-RR)

    FC experiment of HeLa using 83627-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83627-1-RR

    Affinity and Kinetic Characterization of 83627-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83627-1-RR against Human Timp-3 were performed. The affinity constant is 2.63 nM.

Tested Applications

Positive WB detected inhuman placenta tissue
Positive FC (Intra) detected inHeLa cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83627-1-RR is Timp-3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Timp-3 fusion protein Ag34657
Full Name TIMP metallopeptidase inhibitor 3
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24-33 kDa
GenBank Accession NumberBC014277
Gene Symbol TIMP3
Gene ID (NCBI) 7078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Timp-3 (TIMP metallopeptidase inhibitor 3), also known as SFD. It is expected to be located in extracellular space, and the protein is enriched in the placenta tissue and fat tissue. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation, and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. TIMP-3 is expressed as an unglycosylated 24 kDa and glycosylated 29 kDa protein with inhibitory activity against interstitial collagenase, stromelysin-1, and gelatinases A and B (PMID:11827795).

Protocols

Product Specific Protocols
WB protocol for Timp-3 antibody 83627-1-RRDownload protocol
FC protocol for Timp-3 antibody 83627-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols