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STEAP2 Recombinant antibody, PBS Only (Capture)

STEAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240611E5

Cat no : 83611-3-PBS

Synonyms

STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STEAP2 Recombinant antibody (83611-3-RR)

    WB analysis using 83611-3-RR (same clone as 83611-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83611-3-RR (STEAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STEAP2 Recombinant antibody (83611-3-RR)

    IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2-Specific antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STEAP2 Recombinant antibody (83611-3-RR)

    IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2 antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using STEAP2 Recombinant antibody (83611-3-RR)

    FC experiment of A549 using 83611-3-RR (same clone as 83611-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using STEAP2 Recombinant antibody (83611-3-RR)

    FC experiment of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00600-3

    Cytometric bead array standard curve of MP00600-3

    Cytometric bead array standard curve of MP00600-3, STEAP2-Specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83611-3-PBS. Detection antibody: 83611-5-PBS. Standard: SY00725. Range: 0.039-10 ng/mL.

Product Information

83611-3-PBS targets STEAP2 as part of a matched antibody pair:

MP00600-3: 83611-3-PBS capture and 83611-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name six transmembrane epithelial antigen of the prostate 2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberNM_152999
Gene Symbol STEAP2
Gene ID (NCBI) 261729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.