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Dystrophin Recombinant antibody

Dystrophin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240567A3

Cat no : 83609-5-RR

Synonyms

DMD, BMD, 240567A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dystrophin Recombinant antibody (83609-5-RR)

    WB analysis using 83609-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83609-5-RR (DMD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Dystrophin Recombinant antibody (83609-5-RR)

    IHC staining of mouse skeletal muscle using 83609-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83609-5-RR (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Dystrophin Recombinant antibody (83609-5-RR)

    IF Staining of rat brain using 83609-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Dystrophin Recombinant antibody (83609-5-RR)

    IF Staining of rat brain using 83609-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Dystrophin Recombinant antibody (83609-5-RR)

    IF Staining of HepG2 using 83609-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Dystrophin Recombinant antibody (83609-5-RR)

    IF Staining of HepG2 using 83609-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using Dystrophin Recombinant antibody (83609-5-RR)

    FC experiment of HepG2 using 83609-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Dystrophin Recombinant antibody (83609-5-RR, Clone:240567A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83609-5-RR

    Affinity and Kinetic Characterization of 83609-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83609-5-RR against Human Dystrophin were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83609-5-RR is Dystrophin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Dystrophin fusion protein Ag3408
Full Name dystrophin
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight430 kDa, 70 kDa
GenBank Accession NumberBC028720
Gene Symbol Dystrophin
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Protocols

Product Specific Protocols
WB protocol for Dystrophin antibody 83609-5-RRDownload protocol
IHC protocol for Dystrophin antibody 83609-5-RRDownload protocol
IF protocol for Dystrophin antibody 83609-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols