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POMP Recombinant antibody

POMP Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240563D7

Cat no : 83607-6-RR

Synonyms

PNAS 110, hUMP1, HSPC036, HSPC014, C13orf12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POMP Recombinant antibody (83607-6-RR)

    WB analysis using 83607-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using POMP Recombinant antibody (83607-6-RR)

    WB analysis of rat skin using 83607-6-RR

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83607-6-RR

    Affinity and Kinetic Characterization of 83607-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83607-6-RR against Human POMP were performed. The affinity constant is 0.424 nM.

Tested Applications

Positive WB detected inHeLa cells, rat skin tissue, Jurkat cells, HepG2 cells, SW480 cells, HL-60 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83607-6-RR is POMP Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POMP fusion protein Ag6987
Full Name proteasome maturation protein
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003390
Gene Symbol POMP
Gene ID (NCBI) 51371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome.

Protocols

Product Specific Protocols
WB protocol for POMP antibody 83607-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols