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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Cryptochrome 1 Recombinant antibody

Cryptochrome 1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240520B12

Cat no : 83602-5-RR

Synonyms

CRY1, Cryptochrome-1, CRY 1, 240520B12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRY1 Recombinant antibody (83602-5-RR)

    WB analysis using 83602-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83602-5-RR (CRY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83602-5-RR

    Affinity and Kinetic Characterization of 83602-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83602-5-RR against Human Cryptochrome 1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, Y79 cells, HCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83602-5-RR is Cryptochrome 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cryptochrome 1 fusion protein Ag4270
Full Name cryptochrome 1 (photolyase-like)
Calculated Molecular Weight 586 aa, 66 kDa
Observed Molecular Weight60-66 kDa
GenBank Accession NumberBC030519
Gene Symbol CRY1
Gene ID (NCBI) 1407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481).

Protocols

Product Specific Protocols
WB protocol for Cryptochrome 1 antibody 83602-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols