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RUFY3 Recombinant antibody

RUFY3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240519E7

Cat no : 83601-2-RR

Synonyms

RIPx, Rap2-interacting protein x, Rap2 interacting protein x, Protein RUFY3, KIAA0871

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUFY3 Recombinant antibody (83601-2-RR)

    WB analysis using 83601-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using RUFY3 Recombinant antibody (83601-2-RR)

    FC experiment of A431 using 83601-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83601-2-RR

    Affinity and Kinetic Characterization of 83601-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, SGC-7901 cells, HeLa cells, BGC-823 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83601-2-RR is RUFY3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RUFY3 fusion protein Ag35558
Full Name RUN and FYVE domain containing 3
Calculated Molecular Weight 53 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC051716
Gene Symbol RUFY3
Gene ID (NCBI) 22902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).

Protocols

Product Specific Protocols
WB protocol for RUFY3 antibody 83601-2-RRDownload protocol
FC protocol for RUFY3 antibody 83601-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols