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Neurogranin Recombinant antibody

Neurogranin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240526B9

Cat no : 83593-5-RR

Synonyms

NRGN, P17, Ng, NEUG(55-78), hng

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neurogranin Recombinant antibody (83593-5-RR)

    WB analysis using 83593-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83593-5-RR (Neurogranin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using Neurogranin Recombinant antibody (83593-5-RR)

    FC experiment of A549 using 83593-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neurogranin Recombinant antibody (83593-5-RR, Clone:240526B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83593-5-RR

    Affinity and Kinetic Characterization of 83593-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83593-5-RR against Human NRGN were performed. The affinity constant is 0.304 nM.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83593-5-RR is Neurogranin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Neurogranin fusion protein Ag0676
Full Name neurogranin (protein kinase C substrate, RC3)
Calculated Molecular Weight 7.6 kDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberBC002835
Gene Symbol Neurogranin
Gene ID (NCBI) 4900
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.

Protocols

Product Specific Protocols
WB protocol for Neurogranin antibody 83593-5-RRDownload protocol
FC protocol for Neurogranin antibody 83593-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols