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NRGN Recombinant antibody

NRGN Recombinant Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

240526B9

Cat no : 83593-5-RR

Synonyms

Neurogranin, P17, Ng, NEUG(55-78), hng

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRGN Recombinant antibody (83593-5-RR)

    WB analysis using 83593-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83593-5-RR (NRGN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using NRGN Recombinant antibody (83593-5-RR)

    FC experiment of A549 using 83593-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug NRGN Recombinant antibody (83593-5-RR, Clone:240526B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83593-5-RR is NRGN Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRGN fusion protein Ag0676
Full Name neurogranin (protein kinase C substrate, RC3)
Calculated Molecular Weight 7.6 kDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberBC002835
Gene Symbol Neurogranin
Gene ID (NCBI) 4900
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.

Protocols

Product Specific Protocols
WB protocol for NRGN antibody 83593-5-RRDownload protocol
FC protocol for NRGN antibody 83593-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols