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PEBP4 Recombinant antibody

PEBP4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240547D8

Cat no : 83589-5-RR

Synonyms

PEBP 4, hPEBP4, GWTM1933, CORK1, CORK 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PEBP4 Recombinant antibody (83589-5-RR)

    IF Staining of A549 using 83589-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PEBP4 antibody (83589-5-RR, Clone: 240547D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83589-5-RR

    Affinity and Kinetic Characterization of 83589-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83589-5-RR against Human PEBP4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83589-5-RR is PEBP4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEBP4 fusion protein Ag9710
Full Name phosphatidylethanolamine-binding protein 4
Calculated Molecular Weight 223 aa, 25 kDa
GenBank Accession NumberBC020779
Gene Symbol PEBP4
Gene ID (NCBI) 157310
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEBP4 (Phosphatidylethanolamine binding protein 4) is a multifunctional protein that belongs to a small family (PEBP1-4) in mammalian cells. PEBP4 is evolutionally divergent from the other three forms that share almost 80% identity and more than 90% similarity, suggesting that PEBP4 is functionally different from other forms. At cellular levels, it has been shown that the knockdown of PEBP4 in cancer cells suppresses cell proliferation, migration, and invasion, leading to their apoptosis. In contrast, overexpression of PEBP4 induces opposite changes and confers resistance to chemotherapy and radiotherapy (PMID: 35955931).

Protocols

Product Specific Protocols
IF protocol for PEBP4 antibody 83589-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols