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CD320 Recombinant antibody

CD320 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240500D4

Cat no : 83574-3-RR

Synonyms

TCblR, FDC-SM-8D6, FDC-signaling molecule 8D6, 8D6A, 8D6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CD320 Recombinant antibody (83574-3-RR)

    WB analysis of HeLa using 83574-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83574-3-RR (CD320 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83574-3-RR

    Affinity and Kinetic Characterization of 83574-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83574-3-RR against Human CD320 were performed. The affinity constant is 6.55 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, K-562 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83574-3-RR is CD320 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD320 fusion protein Ag21445
Full Name CD320 molecule
Calculated Molecular Weight 29 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC000668
Gene Symbol CD320
Gene ID (NCBI) 51293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 58 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Protocols

Product Specific Protocols
WB protocol for CD320 antibody 83574-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols