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IL-2 Recombinant antibody

IL-2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240416H3

Cat no : 83558-5-RR

Synonyms

IL2, TCGF, Interleukin-2, interleukin 2, IL 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-2 Recombinant antibody (83558-5-RR)

    WB analysis using 83558-5-RR

    Untreated, PMA, A23187 and Brefeldin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83558-5-RR (IL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83558-5-RR

    Affinity and Kinetic Characterization of 83558-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83558-5-RR against Human IL-2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83558-5-RR is IL-2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC070338
Gene Symbol IL2
Gene ID (NCBI) 3558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 83558-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols