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Adiponectin Recombinant antibody

Adiponectin Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240392D6

Cat no : 83525-2-RR

Synonyms

Acdc, Acrp30, Adipoq

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse adipose tissue using Adipoq Recombinant antibody (83525-2-RR)

    WB analysis of mouse adipose using 83525-2-RR

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Adipoq Recombinant antibody (83525-2-RR)

    WB analysis of rat heart using 83525-2-RR

    rat heart tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse adipose tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83525-2-RR is Adiponectin Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name adiponectin, C1Q and collagen domain containing
Calculated Molecular Weight26 kDa
Observed Molecular Weight26-28 kDa
GenBank Accession NumberNM_144744.3
Gene Symbol Adipoq
Gene ID (NCBI) 246253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
WB protocol for Adiponectin antibody 83525-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols