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CKB Recombinant antibody

CKB Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240418D2

Cat no : 83522-5-RR

Synonyms

KCRB, Creatine kinase B-type, CPK-B, CKBB, B-CK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKB Recombinant antibody (83522-5-RR)

    WB analysis using 83522-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CKB Recombinant antibody (83522-5-RR)

    FC experiment of HeLa using 83522-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, Y79 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83522-5-RR is CKB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKB fusion protein Ag7285
Full Name creatine kinase, brain
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC001190
Gene Symbol CKB
Gene ID (NCBI) 1152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

Protocols

Product Specific Protocols
WB protocol for CKB antibody 83522-5-RRDownload protocol
FC protocol for CKB antibody 83522-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols