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  • Western Blot (WB) analysis of human milk using Osteoprotegerin/TNFRSF11B Recombinant antibody (83506-1-RR)

    WB analysis of human milk using 83506-1-RR

    human milk was subjected to SDS PAGE followed by western blot with 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Osteoprotegerin/TNFRSF11B Recombinant antibody (83506-1-RR)

    IHC staining of rat kidney using 83506-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Osteoprotegerin/TNFRSF11B Recombinant antibody (83506-1-RR)

    IHC staining of rat kidney using 83506-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83506-1-RR (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83506-1-RR

    Affinity and Kinetic Characterization of 83506-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83506-1 against Human Osteoprotegerin/ TNFRSF11B were performed. The affinity constant is below 1 pM.

Osteoprotegerin/TNFRSF11B Recombinant antibody

Osteoprotegerin/TNFRSF11B {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA
Cat No. 83506-1-RR
Clone No.240168E9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG, OCIF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman milk
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83506-1-RR targets Osteoprotegerin/TNFRSF11B in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Osteoprotegerin/TNFRSF11B fusion protein Ag2139 Predict reactive species
Full Name tumor necrosis factor receptor superfamily, member 11b
Calculated Molecular Weight 401 aa, 46 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC030155
Gene Symbol TNFRSF11B
Gene ID (NCBI) 4982
RRIDAB_3671131
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00300
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promotes bone formation. OPG plays a key role in cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

Protocols

Product Specific Protocols
WB protocol for Osteoprotegerin/TNFRSF11B antibody 83506-1-RRDownload protocol
IHC protocol for Osteoprotegerin/TNFRSF11B antibody 83506-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols