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Apolipoprotein AI Recombinant antibody

Apolipoprotein AI Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240269F1

Cat no : 83504-1-RR

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    WB analysis of HepG2 using 83504-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83504-1-RR

    Affinity experiments of 83504-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein A1 were performed. The affinity constant is 4.05 nM.

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83504-1-RR is Apolipoprotein AI Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name apolipoprotein A-I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 83504-1-RRDownload protocol
FC protocol for Apolipoprotein AI antibody 83504-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols