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HARS Recombinant antibody

HARS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240495C1

Cat no : 83497-1-RR

Synonyms

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HARS Recombinant antibody (83497-1-RR)

    WB analysis using 83497-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83497-1-RR (HARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using HARS Recombinant antibody (83497-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83497-1-RR (HARS antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using HARS Recombinant antibody (83497-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83497-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83497-1-RR (HARS antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HARS Recombinant antibody (83497-1-RR)

    IF Staining of MCF-7 using 83497-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (83497-1-RR, Clone: 240495C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using HARS Recombinant antibody (83497-1-RR)

    FC experiment of MCF-7 using 83497-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug HARS Recombinant antibody (83497-1-RR, Clone:240495C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using HARS Recombinant antibody (83497-1-RR)

    FC experiment of HeLa using 83497-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HARS Recombinant antibody (83497-1-RR, Clone:240495C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83497-1-RR

    Affinity and Kinetic Characterization of 83497-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83497-1-RR against Human HARS were performed. The affinity constant is 0.382 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83497-1-RR is HARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HARS fusion protein Ag9333
Full Name histidyl-tRNA synthetase
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC011807
Gene Symbol HARS
Gene ID (NCBI) 3035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Protocols

Product Specific Protocols
WB protocol for HARS antibody 83497-1-RRDownload protocol
IHC protocol for HARS antibody 83497-1-RRDownload protocol
IF protocol for HARS antibody 83497-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols