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NCAM2 Recombinant antibody

NCAM2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240398C5

Cat no : 83494-2-RR

Synonyms

NCAM21, NCAM-2, NCAM 2, N CAM 2, 240398C5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAM2 Recombinant antibody (83494-2-RR)

    WB analysis using 83494-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83494-2-RR (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83494-2-RR

    Affinity and Kinetic Characterization of 83494-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83494-2-RR against Human NCAM2 were performed. The affinity constant is 0.882 nM.

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83494-2-RR is NCAM2 Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse , rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCAM2 fusion protein Ag4821
Full Name neural cell adhesion molecule 2
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 90 kDa, 125 kDa
GenBank Accession NumberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

Protocols

Product Specific Protocols
WB protocol for NCAM2 antibody 83494-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols