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RABL2B Recombinant antibody

RABL2B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240501D3

Cat no : 83470-3-RR

Synonyms

Rab-like protein 2B, Rab like protein 2B, 240501D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABL2B Recombinant antibody (83470-3-RR)

    WB analysis using 83470-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using RABL2B Recombinant antibody (83470-3-RR)

    FC experiment of A549 using 83470-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of MCF-7 cells using RABL2B Recombinant antibody (83470-3-RR)

    FC experiment of MCF-7 using 83470-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83470-3-RR

    Affinity and Kinetic Characterization of 83470-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83470-3-RR is RABL2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RABL2B fusion protein Ag2189
Full Name RAB, member of RAS oncogene family-like 2B
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC024281
Gene Symbol RABL2B
Gene ID (NCBI) 11158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).

Protocols

Product Specific Protocols
WB protocol for RABL2B antibody 83470-3-RRDownload protocol
FC protocol for RABL2B antibody 83470-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols