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GSTT2B Recombinant antibody

GSTT2B Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240493G4

Cat no : 83469-4-RR

Synonyms

GST class-theta-2, GST class theta 2, Glutathione S-transferase theta-2B, Glutathione S-transferase theta-2, EC:2.5.1.18

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTT2B Recombinant antibody (83469-4-RR)

    WB analysis using 83469-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83469-4-RR (GSTT2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83469-4-RR

    Affinity experiments of 83469-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83469-4-RR against Human GSTT2B were performed. The affinity constant is below 1 nM.

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse lung tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83469-4-RR is GSTT2B Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSTT2B fusion protein Ag11828
Full Name glutathione S-transferase theta 2B (gene/pseudogene)
Calculated Molecular Weight 244 aa, 28 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC071700
Gene Symbol GSTT2B
Gene ID (NCBI) 653689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTT2B antibody 83469-4-RRDownload protocol
FC protocol for GSTT2B antibody 83469-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols