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TRMT2A Recombinant antibody

TRMT2A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240444C2

Cat no : 83463-8-RR

Synonyms

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT2A Recombinant antibody (83463-8-RR)

    WB analysis using 83463-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-251 cells using TRMT2A Recombinant antibody (83463-8-RR)

    FC experiment of U-251 using 83463-8-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83463-8-RR

    Affinity and Kinetic Characterization of 83463-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

Tested Applications

Positive WB detected inK-562 cells, A431 cells, MCF-7 cells, MOLT-4 cells, HepG2 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83463-8-RR is TRMT2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRMT2A fusion protein Ag9112
Full Name TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
Calculated Molecular Weight 625 aa, 69 kDa
Observed Molecular Weight 42-44 kDa, 68-75 kDa
GenBank Accession NumberBC013352
Gene Symbol TRMT2A
Gene ID (NCBI) 27037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

Protocols

Product Specific Protocols
WB protocol for TRMT2A antibody 83463-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols