• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

POLR2H Recombinant antibody

POLR2H Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240410F9

Cat no : 83439-1-RR

Synonyms

hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, DNA-directed RNA polymerase II subunit H

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using POLR2H Recombinant antibody (83439-1-RR)

    WB analysis using 83439-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83439-1-RR (POLR2H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83439-1-RR

    Affinity and Kinetic Characterization of 83439-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83439-1-RR against Human POLR2H were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83439-1-RR is POLR2H Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POLR2H fusion protein Ag7166
Full Name polymerase (RNA) II (DNA directed) polypeptide H
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC000739
Gene Symbol POLR2H
Gene ID (NCBI) 5437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Protocols

Product Specific Protocols
WB protocol for POLR2H antibody 83439-1-RRDownload protocol
FC protocol for POLR2H antibody 83439-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols