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Conjugate
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Conjugate
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LBP Recombinant antibody

LBP Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230524G10

Cat no : 83427-1-RR

Synonyms

Lipopolysaccharide-binding protein, 230524G10

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using LBP Recombinant antibody (83427-1-RR)

    WB analysis of A431 using 83427-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83427-1-RR (LBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83427-1-RR

    Affinity and Kinetic Characterization of 83427-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83427-1-RR against Human LBP were performed. The affinity constant is 27.1 nM.

Tested Applications

Positive WB detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83427-1-RR is LBP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name lipopolysaccharide binding protein
Calculated Molecular Weight53KD
Observed Molecular Weight 63-67 kDa
GenBank Accession NumberNM_004139.4
Gene Symbol LBP
Gene ID (NCBI) 3929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Protocols

Product Specific Protocols
WB protocol for LBP antibody 83427-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols