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TRNP1 Recombinant antibody

TRNP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240331C6

Cat no : 83410-3-RR

Synonyms

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRNP1 Recombinant antibody (83410-3-RR)

    WB analysis using 83410-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83410-3-RR (TRNP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using TRNP1 Recombinant antibody (83410-3-RR)

    FC experiment of HeLa using 83410-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-3-RR, Clone:240331C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83410-3-RR

    Affinity and Kinetic Characterization of 83410-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-3-RR against Human TRNP1 were performed. The affinity constant is 72.8 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, SH-SY5Y cells, rat heart tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83410-3-RR is TRNP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRNP1 fusion protein Ag22504
Full Name TMF1-regulated nuclear protein 1
Calculated Molecular Weight 227 aa, 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC069216
Gene Symbol TRNP1
Gene ID (NCBI) 388610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

Protocols

Product Specific Protocols
WB protocol for TRNP1 antibody 83410-3-RRDownload protocol
FC protocol for TRNP1 antibody 83410-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols