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AHR Recombinant antibody

AHR Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240325G9

Cat no : 83369-6-RR

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HepG2 using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HeLa using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HeLa using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83369-6-RR is AHR Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AHR fusion protein Ag12193
Full Name aryl hydrocarbon receptor
Calculated Molecular Weight 848 aa, 96 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Protocols

Product Specific Protocols
IF protocol for AHR antibody 83369-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols