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NCAM1/CD56 Recombinant antibody, PBS Only (Capture)

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240078A7

Cat no : 83365-5-PBS

Synonyms

CD56, NCAM1, 240078A7, NCAM 1, NCAM

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00393-4

    Sandwich ELISA standard curve of MP00393-4

    Sandwich ELISA standard curve of MP00393-4, Human NCAM1 Monoclonal Matched Antibody Pair - PBS only. 83365-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31816. 83365-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00393-4

    Sample test of MP00393-4

    The NCAM1 concentration of Human cerebrospinal fluid (CSF) samples were determined to be 127.3 ng/mL.

  • Sample test of MP00393-4

    Sample test of MP00393-4

    Serum of sixteen individual healthy human donors were measured. The NCAM1 concentration of detected samples was determined to be 1.2 ug/mL with a range of 0.7 - 1.9 ug/mL.

Product Information

83365-5-PBS targets NCAM1/CD56 as part of a matched antibody pair:

MP00393-4: 83365-5-PBS capture and 83365-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name neural cell adhesion molecule 1
Calculated Molecular Weight 95 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).