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  • Western Blot (WB) analysis of various lysates using CNKSR2 Recombinant antibody (83354-3-RR)

    WB analysis using 83354-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CNKSR2 Recombinant antibody (83354-3-RR)

    WB analysis using 83354-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83354-3-RR (CNKSR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using CNKSR2 Recombinant antibody (83354-3-RR)

    FC experiment of Jurkat using 83354-3-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CNKSR2 Recombinant antibody (83354-3-RR, Clone:240350D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83354-3-RR

    Affinity and Kinetic Characterization of 83354-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83354-3-RR against Human CNKSR2 were performed. The affinity constant is 11.1 nM.

CNKSR2 Recombinant antibody

CNKSR2 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83354-3-RR
Clone No.240350D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

KSR2, KIAA0902, Connector enhancer of KSR 2, CNK2, CNK homolog protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MDA-MB-231 cells, U-251 cells, mouse brain tissue, U-87 MG cellls, SK-BR-3 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83354-3-RR targets CNKSR2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CNKSR2 fusion protein Ag21174 Predict reactive species
Full Name connector enhancer of kinase suppressor of Ras 2
Calculated Molecular Weight 1034 aa, 118 kDa
Observed Molecular Weight118 kDa, 102 kDa
GenBank Accession NumberBC126121
Gene Symbol CNKSR2
Gene ID (NCBI) 22866
RRIDAB_3671011
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WXI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNKSR2 is a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling.

Protocols

Product Specific Protocols
WB protocol for CNKSR2 antibody 83354-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols