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TCIRG1 Recombinant antibody

TCIRG1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240166B2

Cat no : 83351-6-RR

Synonyms

a3, Atp6i, ATP6N1C, ATP6V0A3, OC 116 kDa, OC 116kDa, OC116, OPTB1, Stv1, T cell immune regulator 1, TCIRG1, TIRC7, V ATPase 116 kDa isoform a3, Vph1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCIRG1 Recombinant antibody (83351-6-RR)

    WB analysis using 83351-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83351-6-RR (TCIRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83351-6-RR

    Affinity and Kinetic Characterization of 83351-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83351-6-RR against Human TCIRG1 were performed. The affinity constant is 2.14 nM.

Tested Applications

Positive WB detected inSKOV-3 cells, HepG2 cells, Jurkat cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83351-6-RR is TCIRG1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCIRG1 fusion protein Ag3378
Full Name T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Calculated Molecular Weight 830 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC018133
Gene Symbol TCIRG1
Gene ID (NCBI) 10312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).

Protocols

Product Specific Protocols
WB protocol for TCIRG1 antibody 83351-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols