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CACHD1 Recombinant antibody

CACHD1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240197A2

Cat no : 83350-4-RR

Synonyms

VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACHD1 Recombinant antibody (83350-4-RR)

    WB analysis using 83350-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83350-4-RR (CACHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83350-4-RR

    Affinity and Kinetic Characterization of 83350-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83350-4-RR against Human CACHD1 were performed. The affinity constant is 6.83 nM.

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83350-4-RR is CACHD1 Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CACHD1 fusion protein Ag22138
Full Name cache domain containing 1
Calculated Molecular Weight 1274 aa, 142 kDa
Observed Molecular Weight 140-170 kDa
GenBank Accession NumberBC039301
Gene Symbol CACHD1
Gene ID (NCBI) 57685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACHD1 (Cache domain-containing protein 1), also called VWCD1 (VWFA and cache domain-containing protein 1), is a type I membrane protein involved that modulates the activities of voltage-gated Ca(2+) channels (VGCCs) (PMID: 30181139; 30404013). CACHD1 has two isoforms, with molecular weights of 142 kDa and 109 kDa respectively. It has certain glycosylation modifications, and the molecular weight of isoform1 after glycosylation modification is 170 kDa (PMID: 30983497; 30404013). This antibody recognizes 142-170 kDa.

Protocols

Product Specific Protocols
WB protocol for CACHD1 antibody 83350-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols