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IGFBP1 Recombinant antibody, PBS Only (Capture)

IGFBP1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240263B5

Cat no : 83347-2-PBS

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00355-3

    Sandwich ELISA standard curve of MP00355-3

    Sandwich ELISA standard curve of MP00355-3, Human IGFBP1 Recombinant Matched Antibody Pair - PBS only. 83347-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0951. 83347-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    Serum of eight individual healthy human donors was measured. The IGFBP1 concentration of detected samples was determined to be 18,764.2 pg/mL with a range of 1,025.1 - 37,369.6 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IGFBP1, and measured 43,726.4 pg/mL.

  • Sample test of MP00355-3

    Sample test of MP00355-3

    The mean human IGFBP1 concentration was determined to be 38,698.4 pg/mL in HepG2 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP00355-3

    Cytometric bead array standard curve of MP00355-3

    Cytometric bead array standard curve of MP00355-3, IGFBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83347-2-PBS. Detection antibody: 83347-4-PBS. Standard: Eg0951. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00355-1

    Cytometric bead array standard curve of MP00355-1

    Cytometric bead array standard curve of MP00355-1, IGFBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83347-2-PBS. Detection antibody: 83347-3-PBS. Standard: Eg0951. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00355-2

    Cytometric bead array standard curve of MP00355-2

    Cytometric bead array standard curve of MP00355-2, IGFBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83347-2-PBS. Detection antibody: 83347-1-PBS. Standard: Eg0951. Range: 0.313-40 ng/mL.

Product Information

83347-2-PBS targets IGFBP1 as part of a matched antibody pair:

MP00355-1: 83347-2-PBS capture and 83347-3-PBS detection (validated in Cytometric bead array)

MP00355-2: 83347-2-PBS capture and 83347-1-PBS detection (validated in Cytometric bead array)

MP00355-3: 83347-2-PBS capture and 83347-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name IGF binding protein 1
Calculated Molecular Weight28 kDa
GenBank Accession NumberNM_000596.4
Gene Symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.