• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CRIP1 Recombinant antibody

CRIP1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240176G2

Cat no : 83340-3-RR

Synonyms

CRP1, CRP 1, CRIP 1, CRIP, CRHP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CRIP1 Recombinant antibody (83340-3-RR)

    WB analysis using 83340-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83340-3-RR (CRIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using CRIP1 Recombinant antibody (83340-3-RR)

    IHC staining of human colon using 83340-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83340-3-RR (CRIP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83340-3-RR

    Affinity and Kinetic Characterization of 83340-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83340-3-RR against Human CRIP1 were performed. The affinity constant is 0.784 nM.

Tested Applications

Positive WB detected inT-47D cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83340-3-RR is CRIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CRIP1 fusion protein Ag7588
Full Name cysteine-rich protein 1 (intestinal)
Calculated Molecular Weight 9 kDa
Observed Molecular Weight 8-9 kDa
GenBank Accession NumberBC002738
Gene Symbol CRIP1
Gene ID (NCBI) 1396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).

Protocols

Product Specific Protocols
WB protocol for CRIP1 antibody 83340-3-RRDownload protocol
IHC protocol for CRIP1 antibody 83340-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols