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DNase I Recombinant antibody

DNase I Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240222F11

Cat no : 83337-6-RR

Synonyms

DNASE1, EC:3.1.21.1, DRNI, DNL1, Deoxyribonuclease-1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using DNase I Recombinant antibody (83337-6-RR)

    WB analysis of mouse heart using 83337-6-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83337-6-RR (DNase I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human urine sample using DNase I Recombinant antibody (83337-6-RR)

    WB analysis of human urine using 83337-6-RR

    human urine sample were subjected to SDS PAGE followed by western blot with 83337-6-RR (DNase I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83337-6-RR

    Affinity and Kinetic Characterization of 83337-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83337-6-RR against Human DNase I were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inmouse heart tissue, human urine sample

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83337-6-RR is DNase I Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNase I fusion protein Ag10117
Full Name deoxyribonuclease I
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31-40 kDa
GenBank Accession NumberBC029437
Gene Symbol DNASE1
Gene ID (NCBI) 1773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).

Protocols

Product Specific Protocols
WB protocol for DNase I antibody 83337-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols