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  • Featured Product
  • KD/KO Validated

GANAB Recombinant antibody

GANAB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240279B7

Cat no : 83324-1-RR

Synonyms

Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GANAB Recombinant antibody (83324-1-RR)

    WB analysis using 83324-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    WB analysis of HeLa using 83324-1-RR

    WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GANAB Recombinant antibody (83324-1-RR)

    IHC staining of mouse liver using 83324-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    IF Staining of HeLa using 83324-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    FC experiment of HeLa using 83324-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83324-1-RR

    Affinity and Kinetic Characterization of 83324-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.

Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, human placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83324-1-RR is GANAB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GANAB fusion protein Ag30417
Full Name glucosidase, alpha; neutral AB
Calculated Molecular Weight944aa,107 kDa; 488aa,55 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC017435
Gene Symbol GANAB
Gene ID (NCBI) 23193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GANAB antibody 83324-1-RRDownload protocol
IHC protocol for GANAB antibody 83324-1-RRDownload protocol
IF protocol for GANAB antibody 83324-1-RRDownload protocol
FC protocol for GANAB antibody 83324-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols