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AQP6 Recombinant antibody

AQP6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240175F9

Cat no : 83322-3-RR

Synonyms

Aquaporin6, Aquaporin 6, AQP 6, 240175F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AQP6 Recombinant antibody (83322-3-RR)

    WB analysis using 83322-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83322-3-RR (AQP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83322-3-RR

    Affinity and Kinetic Characterization of 83322-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83322-3-RR against Human AQP6 were performed. The affinity constant is 0.749 nM.

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, MCF-7 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83322-3-RR is AQP6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AQP6 fusion protein Ag26845
Full Name aquaporin 6, kidney specific
Calculated Molecular Weight 29 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberNM_001652
Gene Symbol AQP6
Gene ID (NCBI) 363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP6 (Aquaporin-6) has recently been identified as an intracellular vesicle water channel with anion permeability that is activated by low pH or HgCl2 (PMID: 12034750). It could be involved in physiological mechanisms of fluid movement, acid-base regulation, and/or anion transport. The calculated MW of AQP6 is 29 kDa, but the actual observed MW is around 35 kDa, which represents glycosylated AQP6 monomer forms (PMID: 19811639).

Protocols

Product Specific Protocols
WB protocol for AQP6 antibody 83322-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols