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EGLN3 Recombinant antibody

EGLN3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240165A6

Cat no : 83321-1-RR

Synonyms

PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3, EC:1.14.11.29

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EGLN3 Recombinant antibody (83321-1-RR)

    IF Staining of HepG2 using 83321-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3 antibody (83321-1-RR, Clone: 240165A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using EGLN3 Recombinant antibody (83321-1-RR)

    FC experiment of MCF-7 using 83321-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using EGLN3 Recombinant antibody (83321-1-RR)

    FC experiment of A549 using 83321-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83321-1-RR

    Affinity and Kinetic Characterization of 83321-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83321-1-RR against Human EGLN3 were performed. The affinity constant is 1.82 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83321-1-RR is EGLN3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EGLN3 fusion protein Ag27464
Full Name egl nine homolog 3 (C. elegans)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC010992
Gene Symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
IF protocol for EGLN3 antibody 83321-1-RRDownload protocol
FC protocol for EGLN3 antibody 83321-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols