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APIP Recombinant antibody

APIP Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240189E2

Cat no : 83320-5-RR

Synonyms

APAF1 interacting protein, APIP, APIP2, CGI 29, CGI29, dJ179L10.2, MMRP19, MTRu 1 P dehydratase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APIP Recombinant antibody (83320-5-RR)

    WB analysis using 83320-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83320-5-RR (APIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83320-5-RR

    Affinity and Kinetic Characterization of 83320-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83320-5-RR against Human APIP were performed. The affinity constant is 5.86 nM.

Tested Applications

Positive WB detected inPC-3 cells, K-562 cells，

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83320-5-RR is APIP Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APIP fusion protein Ag2729
Full Name APAF1 interacting protein
Calculated Molecular Weight 242 aa, 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC009077
Gene Symbol APIP
Gene ID (NCBI) 51074
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APIP antibody 83320-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols