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ADRB2 Recombinant antibody

ADRB2 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240186C9

Cat no : 83317-5-RR

Synonyms

ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ADRB2 Recombinant antibody (83317-5-RR)

    IF Staining of A431 using 83317-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ADRB2 antibody (83317-5-RR, Clone: 240186C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 83317-5-RR

    Affinity experiments of 83317-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83317-5-RR against Human ADRB2 were performed. The affinity constant is 53.9 pM.

Tested Applications

Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83317-5-RR is ADRB2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADRB2 fusion protein Ag3715
Full Name adrenergic, beta-2-, receptor, surface
Calculated Molecular Weight46 kDa
GenBank Accession NumberBC012481
Gene Symbol ADRB2
Gene ID (NCBI) 154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity, and type 2 diabetes.

Protocols

Product Specific Protocols
IF protocol for ADRB2 antibody 83317-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols