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NUPR1 Recombinant antibody

NUPR1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240149G4

Cat no : 83315-2-RR

Synonyms

Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8

Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using NUPR1 Recombinant antibody (83315-2-RR)

    WB analysis of NCI-H1299 using 83315-2-RR

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 83315-2-RR (NUPR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83315-2-RR is NUPR1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUPR1 fusion protein Ag7039
Full Name nuclear protein 1
Calculated Molecular Weight 8 kDa
Observed Molecular Weight10 kDa
GenBank Accession NumberBC002434
Gene Symbol NUPR1
Gene ID (NCBI) 26471
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa, it also has another isoform of 10 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

Protocols

Product Specific Protocols
WB protocol for NUPR1 antibody 83315-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols